摘要
为评价青枯灵的环境安全性及指导科学合理使用青枯灵,建立了青枯灵在水稻中的残留量分析方法.水稻土壤、稻壳、稻草、糙米、植株中青枯灵残留量采用甲醇:水混合液提取,采用三氯甲烷萃取(稻田水直接用三氯甲烷萃取),萃取液浓缩后过弗罗里硅土柱净化,淋出液经浓缩后再用甲醇定容,HPLC检测.当添加0.05～5.0mg/kg时,样品的平均回收率为79.23%～103.19%,变异系数为1.51%～9.33%.方法最小检出量为1.82×10-10g,稻田水、土壤、糙米、稻壳、稻草、植株的最低检测质量浓度分别为0.001 mg/L,0.005,0.01,0.03,0.03,0.03 mg/kg.
In order to assess environmental safety of Qingkuling and make a scientific use of it,residual analytical method of Qingkuling in rice was established.The residue of Qingkuling in soil,rice hull,rice stalk,rice and plant samples was extracted with methanol-water mixture,which was reextracted with trichloromethane(water sample was reextracted with trichloromethane directly).After being concentrated by evaporation,the reextract were cleaned up by a column chromatography on florisil.The final residue could be detected by HPLC with high sensitivity.The average recoveries from untreated control samples fortified with Qingkuling at quality levels of 0.05 to 5.0 mg/kg ranged from 79.23% to 103.19%,and the coefficients of variation were from 1.51% to 9.33%.The lowest detectable quality was 1.82×10─10 g and the lowest detectable concentrations of various samples were 0.001 mg/L of water,0.005 mg/kg of soil,0.01 mg/kg of rice,0.03 mg/kg of rice hull,0.03 mg/kg of stalk and 0.03 mg/kg of plant respectively.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第3期320-323,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
农业部资助项目(2002051)
关键词
青枯灵
液相色谱
残留
水稻
Qingkuling
liquid chromatography
residue
rice
