摘要
背景:脑缺血时,抑制神经细胞凋亡的Bcl-2基因和诱导细胞凋亡的Bax均参与了其发病过程。扇贝多肽的体外抗氧化和抗凋亡作用,是否可产生对抗脑缺血后中枢神经细胞凋亡的保护作用?目的:观察扇贝多肽对大鼠脑缺血半影区内Bcl-2和Bax蛋白参与的细胞凋亡的抑制及其脑保护作用。设计:随机对照实验。单位:青岛大学医学院的人体解剖学教研室。材料:实验于2000-03/04在青岛大学医学院神经解剖实验室完成。选择成年Wistar大鼠32只,随机分为扇贝多肽组、蒸馏水组、模型对照组、假手术组,8只/组。扇贝多肽由中国水产科学院黄海水产研究所提供。干预:扇贝多肽组、蒸馏水组和模型对照组采用颈外动脉线栓法建立缺血再灌注模型,假手术组不予造模。扇贝多肽组术前2d腹腔注射体积分数0.1的扇贝多肽0.1mL/kg,1次/d,再灌注前15min加注1次;蒸馏水组术前2d腹腔注射双蒸水0.1mL/kg,1次/d,再灌注前15min加注1次;模型对照组和假手术组术前不给药。然后扇贝多肽组、蒸馏水组和模型对照组开始造模,从颈外动脉插入4-0尼龙线,经颈总动脉分叉处、颈内动脉颅外段进入颈内动脉颅内段到达大脑中动脉分支处阻塞大脑中动脉的起始部,引起大脑中动脉供血区的急性脑缺血。动物苏醒后出现左霍纳综合征,提尾时右前肢内收屈曲,爬行时向右划圈为造模成功。假手术组手术过程不闭塞大脑中动脉,其余步骤同以上3组。将各组大鼠断头取脑,进行免疫组化Bcl-2和Bax蛋白的测定,结果用免疫反应产物吸光度(A)值来表示Bcl-2和Bax蛋白在脑组织缺血半影区的表达水平。主要观察指标:各组大鼠脑组织缺血半影区Bcl-2和Bax蛋白表达的差异。结果:经补充后32只大鼠进入结果分析。①缺血半影区Bcl-2蛋白的表达:模型对照组及蒸馏水组吸光度值明显高于假手术组[0.453±0.048,0.510±0.061,0.211±0.023(F=683.7
BACKGROUND:Both apoptosis suppression gene Bcl2 and apoptosis induction gene Bax take parts in the apoptosis of neurons in ischemic penumbra.Whether would the polypeptide from chlamys farreri that is proved to be of antioxidation and antiapoptosis in vitro protect the ischemic neurons from apoptosis?OBJECTIVE:To observe the effect of chlamys farreri on the Bcl2 and Bax proteinassociated apoptosis in penumbra and its role in neuron protection.DESIGN:A randomized trial.SETTING:Department of Anatomy,Medical College of Qingdao University.MATERIALS:The trial was conducted in Nerve Anatomy Laboratory of Medical College of Qingdao University from March to April 2000.The subjects were 32 adult Wistar rats that were randomly and averagely assigned into 4 groups:polypeptide chlamys farreri group,sterile water group,model control group and sham group.The chlamys farreri was provided by the Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences. INTERVENTIONS:Model of brain ischemia and reperfusion was made in rats in polypeptide chlamys farreri,sterile water and model control group by occlusion of middle cerebral artery.The model was not established in rats in sham group.The rats in chlamys farreri group received intraperitoneal injection of chlamys farreri of volume fraction 0.1 at the dose of 0.1 mL/kg each day for 2 days prior to modeling and an extra injection 15 minutes just before modeling.And the rats in sterile water group received intraperitoneal injection of sterile water with the dose of 0.1 mL/kg each for two days and an extra injection 15 minutes before modeling.Those in model control group and sham group were exposed to nothing. Then models were established in rats in chlamys farreri,sterile water and model control group by inserting 40 nylons sutures from external carotid artery through bifurcation of carotid artery, extracranial and intracranial segments of internal carotid artery till the initial part of middle cerebral artery to make acute ischemia of middle cerebral
出处
《中国临床康复》
CSCD
北大核心
2005年第21期234-235,共2页
Chinese Journal of Clinical Rehabilitation
