摘要
参考已发表的番鸭呼肠孤病毒(mDRV)的S4序列设计了1对扩增σC蛋白基因的引物,对mDRVZJ99株的RNA抽提物进行RTPCR扩增,获得了特异性的扩增片段。将PCR产物克隆测序,序列经同源性比较,发现mDRVZJ99株σC蛋白基因序列与福建MW9710株对应基因的同源性为99.5%,与法国89026株对应基因的同源性为94.2%,与法国89330株对应基因的同源性为95.0%;相应的氨基酸序列同源性分别为98.9%、94.1%、93.7%。基于σC基因及其推导氨基酸序列的蛋白质结构及物理化学性质预测结果,与国外学者对mDRVσC蛋白的报道相似。
A pair of primers for amplification of σC gene was designed and synthesized according to the published Muscovy duck reovirus (mDRV) S4 gene sequence. The specific RT-PCR product was successfully obtained and cloned from the Muscovy duck reovirus ZJ99 strain. The results showed that the σC gene of mDRV ZJ99 had 99.5% homology with that of mDRV MW9710 isolated in Fujian Province of China, 94.2% and 95.0% homology with that of mDRV 89026 and that of mDRV 89330 reported in France respectively. The homology of the deduced amino acid sequences were 98.9%, 94.1% and ~93.7% , correspondingly. The predicted physicaland chemical characters andstructures of the σC protein of mDRV ZJ99 were similar to that reported by Kuntz-Simon from France.
出处
《中国兽医科技》
CAS
CSCD
北大核心
2005年第6期428-431,共4页
Chinese Journal of Veterinary Science and Technology
基金
浙江省科技计划重点项目(011102120)
