摘要
用解吸-热提两步提取法(简称两步法)提取银杏(Ginkgobiloba)叶中的黄酮,并与传统的醇提和水提方法进行比较。两步法是在10.00g银杏叶粉末中加入解吸剂乙醇溶液,25℃静置一定时间,再加入沸腾的提取溶剂乙醇溶液提取,提取2次,合并滤液进行分析。结果表明,两步法提取银杏黄酮的最佳操作参数为解吸剂乙醇浓度为60%、用量为样品量的1.6倍,解吸时间15min,溶剂乙醇浓度50%,提取2次,每次15min、溶剂用量为样品量的40倍。两步法提取黄酮的得率为2.69%,比水提法提高了0.57%,比醇提法略高;两步法提取的浸膏黄酮含量为6.91%,比水提法提高了1.89%、比醇提法提高了0.96%;两步法的提取时间比传统法少4～8倍。
Flavonoids in Ginkgo biloba was extracted by the method of two-steps of release and hot-extraction(two-steps).This method was compared to extracting with ethanol and extracting with water.In two-steps,first,10.00g Ginkgo biloba was put little release solvent under 25℃ to keep sometime.And then,boiling extraction solvent of ethanol solution was added.The filtrates from two times extracting were mixed and analyzed.The result showed that the best conditions of two-steps were 60% 16 ml ethanol solution using as release solvent,15 min extracting time for every time and solvent quantity which was 40 times of the weight of sample.The extraction ratio of the two-steps was 2.69%.This result was 1.27 times than extracting with water and equal to extracting with ethanol.The extraction content of the two-steps was 6.91% in extract.This result was 1.38 times than extracting with water in extract 1.15 times than extracting with water in extract.The extraction time of two-steps was only 1/4 and 1/8 of traditional methods.
出处
《广西科学》
CAS
2005年第2期127-129,132,共4页
Guangxi Sciences
基金
广西大学科学技术研究重点基金项目(编号:2003ZD05)资助。
关键词
银杏
黄酮
提取
两步法
Ginkgo biloba,flavonoids,extraction,two-steps
