摘要
目的:研究蛋白酶激活受体(PAR)2激动剂(SLIGKV和tc LIGRLO)、胰蛋白酶及其抑制剂(SBTI、α1AT)对A549肺上皮细胞胞浆内钙离子浓度([Ca2+]i)的影响。方法:用Fluo3AM荧光标记技术和激光扫描共聚焦显微镜检测不同因素处理的A549肺上皮细胞[Ca2+]i。结果:胰蛋白酶、SLIGKV、tc LIGRLO均能引发A549肺上皮细胞[Ca2+]i的增加,平均荧光强度分别比加入试剂前增加92%,47%和130%。SBTI和α1AT可抑制胰蛋白酶诱导的细胞[Ca2+]i的增加。结论:PAR2可介导A549肺上皮细胞[Ca2+]i的释放增加,SBTI和α1AT可抑制胰蛋白酶诱导的细胞[Ca2+]i的增加。
Objective:To study the effects of protease-activated receptor-2(PAR-2)agonists(SLIGKV and tc-LIGRLO),trypsin and its inhibitors[soybean trypsin inhibitor(SBTI)and α_1-antitrypsin(α_1-AT)]on intracellular calcium concentration([Ca^(2+)]_i)in A549 lung epithelial cell.Methods:Fluo-3/AM fluorescence technique and laser scanning confocal microscope were used to measure the intracellular calcium level after different reagents were added in Hanks of A549 cells. Results: Trypsin as well as SLIGKV, tc-LIGRLO could increase the[Ca^(2+)]_i in A549 cells. Compared with Hanks alone, the average fluorescent intensities were increased 92%,47%,130% respectively when the reagents were added. SBTI and α_1-AT could inhibit the increase of[Ca^(2+)]_i induced by trypsin in A549 cells. Conclusion:Activation of PAR-2 can induce the increase of[Ca^(2+)]_i in A549 cells. SBTI and α_1-AT can inhibit the increase of[Ca^(2+)]_i induced by trypsin in A549 cells.
出处
《汕头大学医学院学报》
2005年第2期81-82,85,共3页
Journal of Shantou University Medical College
基金
李嘉诚基金资助项目(C0200001)
