摘要
为了研究伴侣分子相互作用蛋白CHIP对TGF-β信号通路的调控,利用四环素基因表达调控系统,建立四环素调控表达CHIP的稳定细胞系(Mv1Lu-Tetoff-CHIP).利用此细胞模型,发现CHIP的过量表达可显著降低细胞内Smad2/3蛋白水平;荧光素酶报告分析也表明开启CHIP蛋白表达可明显降低Smads介导的基因转录活性;进一步的免疫印迹结果显示CHIP蛋白可明显下调TGF-β所诱导的下游基因JunB的表达.上述结果提示CHIP可以作为一种新的蛋白质分子抑制性调节TGF-β信号通路.
To investigate the regulatory function of chaperone interacting protein CHIP in TGF-β signal pathway, a tetracycline induced CHIP expression stable cell line (Mv1Lu-Tet off-CHIP) was generated. In this cell line model, it was observed that overexpression of CHIP dramatically reduced the endougenous Smad2/3 protein level. Luciferase reporter analysis showed that CHIP inhibited the Smads induced transcription activity. Furthermore, Western blot results indicated that CHIP could downregulate gene expression of JunB, a quick response gene induced by TGF-β. These results provided new evidences that CHIP might be a novel signal protein negatively regulating TGF-β signal pathway.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2005年第6期557-561,共5页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目(30470888)
国家重点基础研究发展规划项目(973)(2001CB510006
2002CB51300)
裕元基金资助项目(20240005-06)~~
