摘要
目的:探讨对氧磷酯酶2S311C多态性与2型糖尿病及糖尿病肾病发生的关系。方法:选择2003-05/2004-09解放军第四军医大学唐都医院内分泌科住院的2型糖尿病患者94例(糖尿病组),其中包括糖尿病肾病患者29例(剔除有尿蛋白和高血压而无视网膜病变患者17例),未伴发糖尿病肾病患者42例(剔除了尿白蛋白阴性但同时有糖尿病视网膜等微血管病变患者6例)。选择同期本院体检中心体检健康自愿者45人为对照组。运用荧光偏振-模板依赖的染料掺入反应法技术检测3组对象对氧磷酯酶2S311C基因多态性的等位基因和基因型分布频率。结果:按意向处理分析,进入结果分析糖尿病患者94例,健康体检者45人。为了分析糖尿病肾病与对氧磷酯酶2C311等位基因,进入结果分析的糖尿病肾病患者29例,未伴发糖尿病肾病患者42例。①糖尿病组对氧磷酯酶2C311等位基因频率显著高于对照组(43.6%,31.1%,χ2=3.981,P<0.05,OR=1.713,95%CI:1.007～2.914)。②糖尿病肾病与未伴发糖尿病肾病患者C311等位基因及基因型分布无明显差异(P>0.05)。③Logistic回归显示,收缩压、三酰甘油升高是2型糖尿病发生的危险因素,而对氧磷酯酶2C311等位基因(P=0.106)未进入回归方程。结论:对氧磷酯酶2C311等位基因与2型糖尿病患病相关联,为其微效基因,而与糖尿病肾病并发症的发生无关。
AIM:To investigate the relationship between paraoxonase 2 S311C gene polymorphism and the onset of type 2 diabetes mellitus and diabetic nephropathy.METHODS:Ninety-four inpatients with type 2 diabetes mellitus,including 29 cases of diabetic nephropathy,excluding 17 cases who had proteinuria and hypertention without diabetic retinopathy,and 42 cases not accompanied by diabetic nephropathy,excluding 6 patients who had no proteinuria but had diabetic retinopathy,were selected from the Department of Endocrinology,Tangdu Hospital,Fourth Military Medical University of Chinese PLA between May 2003 and September 2004.Forty five healthy physical examinees in the physical examination center of this hospital at the same period were voluntarily taken as the controls(control group).The alleles and genotypes distribution frequencies of paraoxonase 2 S311C gene polymorphism in the 3 groups were detected with the by template directed dye terminator incorporation with fluorescence polarization(TDI FP).RESULTS:According to intention to treat analysis,all the 94 diabetic patients and 45 healthy physical examinees were involved in the analysis of results.In order to analyze diabetic nephropathy and paraoxonase 2 S311C allele,29 patients with diabetic nephropathy and 42 cases not accompanied by diabetic nephropathy were involved in the analysis of results.① The frequency of paraoxonase 2 311C allele was significantly higher in the diabetic group than in the control group(43.6% ,31.1% ,χ 2=3.981,P< 0.05,OR=1.713, 95% confidential interval:1.007 to 2.914).② There were no significant difference in paraoxonase 2 S311C allele and genotype distribution between patients with simple diabetic nephropathy and patients not accompanied by diabetic nephropathy(P >0.05).③ Logistic regression showed that the increases of systolic pressure and triacylglycerol were the risk factors for the occurrence of type 2 diabetes mellitus,but paraoxonase 2 311C allele did not enter the regression equation.CONCLUSION:Paraoxonase 2 C311 allele is associate
出处
《中国临床康复》
CSCD
北大核心
2005年第19期126-128,共3页
Chinese Journal of Clinical Rehabilitation
基金
陕西省科技攻关课题项目[2002k10-G7(04)]~~
