摘要
将口蹄疫O型抗原决定簇和A型抗原决定簇构成的融合基因O21O14A21经农杆菌介导转入粳稻品种日本晴中,经潮霉素筛选,获得再生植株207株.提取它们的总DNA进行PCR检测,其中193株扩增出和阳性对照相同的900bp和450bp两条带,转基因阳性率达93.2%,抽取部分阳性植株进行PCRSouthern杂交检测及报告基因GUS检测,同样证明融合基因已经整合到日本晴基因组中.
Plant-derived vaccines are becoming one focus of plant gene engineering,which has such advantages as easy production,low cost,convenience and safe use and easy storage.In this experiment,fused genes O_21 -O_14 -A_21 containing both type O and A FMDV antigenic determinant were introduced into rice (Oryza Sativa Japonica cultivar Npponbare ) mediated by Agrobacterium.Selected by Hyg 207 independent seedlings regenerated from transformants have already been obtained.Their total DNA extracted and detected by PCR,193 samples (93.2% of all) amplified the 450 bp and 900 bp two strips same as the positive contrast.Detection of partial PCR-positive seedlings by GUS and PCR-Southern blotting show that they are all positive,which also revealed that the O_21 -O_14 -A_21 gene has transfered to rice genomes.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第B06期158-161,共4页
Journal of Xiamen University：Natural Science
基金
国家科技部转基因专项
福建省科技厅重点项目资助
