摘要
在构建口蹄疫病毒(FootandMouthDiseaseVirus,FMDV)结构蛋白P1基因植物表达载体 pBI131SP1的基础上 ,以玉米自交系8902、340和4112的Ⅱ型胚性愈伤组织为受体 ,用基因枪轰击法转化玉米 ,获得抗性再生植株。GUS染色证明外源目的基因在玉米细胞和组织中得到了表达。PCR检测、PCR -Southern杂交、点杂交鉴定证实目的基因P1已整合到再生植株的基因组中 ,获得了转基因玉米再生植株。
Plant expression vector pBI131SP1was constructed with P1gene of FMDV.TypeⅡcalli were initiated from immature emˉbryoes of maize inbred lines8902,340and4112,and were transformed with particle bombardment.Kanamycin-resistant regenerated plants had been obtained in this experiment.GUS assay indicated that foreign gene had expressed in maize cell and calli.It was demonstrated that P1gene had been inserted into maize genome after PCR,PCR-Southern blotting and dot blotting analysis of regenˉerated plants.The transgenic maize regenerated plants have been obtained.
出处
《沈阳农业大学学报》
CAS
CSCD
2003年第6期423-425,共3页
Journal of Shenyang Agricultural University
基金
863资助项目(2001AA213071)
国家植物转基因中试及产业化基地专项课题(J99B001)
