摘要
建立了一种同步荧光检测虎杖提取物中白藜芦醇含量的新方法,系统地研究了检测条件对白藜芦醇荧光强度的影响,以及实验的准确性、重现性、回收率.该方法的线性回归方程为y=18.90x+3.129 8,荧光强度与浓度在0～1.68×10-5mol/L的范围内具有良好的线性关系,检出限为8.839×10-10mol/L.研究表明,该检测方法操作简便、可行、成本低,检测结果较为准确,能满足科研和工业生产的要求.
A new method was established to determine content of resveratrol in extract of Polygonum cuspidatum Siab. et Zucc. by sychronous fluorometry. Effects of fluoresence intensity, as well as its precision, repetition and recovery efficiency were studied systematically. The linear relationship was y = 18.90x + 3.129 8,or fluorescence intensity of the system presented a linear relationship with concentration of resveratrol in the range of 0-1.68×10 -5 mol/L, the detection limit was 8.839×10 -10 mol/L.The study indicated that this method is simple, viable, of low cost and high precision. It could be used in both scientific research and industrial production.
出处
《林产化学与工业》
EI
CAS
CSCD
2005年第2期63-66,共4页
Chemistry and Industry of Forest Products
基金
湖南省科技厅重点科技攻关资助项目(03JZY3020)
关键词
荧光分析法
白藜芦醇
虎杖
fluorometry
resveratrol
Polygonum cuspidatum Siab. et Zucc.
