摘要
用免疫组织化学（ＡＢＣ）方法结合图象分析和脑干电反应测听技术（ＡＢＲ），研究冲击波对豚鼠耳蜗血管纹（ＳＶ）、螺旋神经节（ＳＧ）心钠素免疫性反应（ＡＮＰ－ＩＲ）影响及其与听阈阈移的关系。结果表明，冲击波暴露后６、１２、２４、４８小时，ＳＶ和ＳＧ中ＡＮＰ－ＩＲ产物的光密度值较７２小时组和对照组明显增高（Ｐ＜０．０１），７２小时与其对照则无显著差异（Ｐ＞０．０５）。ＳＶ和ＳＧ中ＡＮＰ－ＩＲ光密度值的变化与ＡＢＲ阈移均呈现正相关。提示压力波暴露后血管纹中的ＡＮＰ的增高可能是内耳对压力波所致听力损伤的一种代偿机制；而螺旋神经节细胞中ＡＮＰ－ＩＲ含量的变化可能是压力波所致听觉通路损害的一种病理表现。探讨了ＡＮＰ在压力波所致听力损伤中可能起的保护作用，以及压力波对ＳＶ、ＳＧ中ＡＮＰ－ＩＲ的影响。
The effect of pressure wave on immunoreactive atrial natriceretic peptides (ANP) in the
cochlea stria vascularis and spiral gan-glion of guinea pigs was studied by ABC
immunohistochemistry, image analysis and auditory brainstem response. The result
showedthat a 6,12 24, 48 hours after pressure wave explosure. the ANP～IR optic density was
higher than that of 72 h group and the con-trol in the stria vascularis and spiral ganglion
(P<0.01). There was no difference between the 72 h and the control groups (P>0.05). ANp-IR optic
density in the stria vascularis and spiral ganglion was directly correlated with ABR threshold
shift (r1=0.8109,r2 =0.9385,The result suggested that the increased ANP in the stria vascularis
may be compensatory mechanism of hearing losscaused by pressure wave :the increased ANP
in the spiral ganglion may be a pathological manifestation of auditory pathway causedby
pressure wave.The protective function of in the stria vascularis may be compensatory
mechanism of hearing loss eaused by pres-sure wave: the increased ANP in hearing loses
caused by pressure wave and the effect of pressure wave to ANP-IR of the stria vas-cular and
spiral ganglion are discussed.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1994年第6期271-272,共2页
Chinese Journal of Trauma
基金
国家自然科学基金
