摘要
用免疫组织化学（ＡＢＣ）方法结合图象分析和听觉电生理技术研究了冲击波对豚鼠耳蜗血管纹心钠素（ＡＮＰ）的影响及其与听阈阈移的关系。结果表明：冲击彼暴露后６、１２、２４、４８小时组，血管纹中ＡＮＰ－ＩＲ产物的光密度值较７２小时组和对照组明显增高（Ｐ＜０．０１），７２小时与对照组的光密度值无显著差异（Ｐ＞０．０５）。血管纹中ＡＮＰ－ＩＲ光密度值的变化与ＡＢＲ阈移呈正相关。提示，血管纹中的ＡＮＰ含量的增高可能是机体对冲击波所致听力损伤的一种代偿机制；ＡＮＰ在冲击波所致听力损伤中可能起着保护作用。
The effect of shock wave on immunoreactive atrial natriceretic peptides(ANP) in the cochlea stria vascularis and spiral ganglion of guinea pigs were studied by ABC immunocytochemistry, image analysis and auditory electrophysiology. The results showed that 6h, 12h, 24h,48h after shock wave explosure,the ANp-IR optic densities were higher than those of 72h and controls (P<0. 01). There were no differences between the 72h and cotrol groups(P>0.05). ANp-IRoptic density were directly correlated with ABR threshold shift(Rx=0. 8109,Rx=0. 9385). Theresults suggested that the increased ANP in the stria vascularis may be a compensatory mechanism to hearing lose caused by shock wave;the increased ANP in the spiral ganglion may be a pathological manifestation of auditory pathway caused by shock wave, The protective function of ANP inhearing lose caused by shock wave and the effect of shock wave to ANP-IR of the stria vascular and spiral ganglion were discussed.
出处
《临床耳鼻咽喉科杂志》
CSCD
1994年第2期99-101,共3页
Journal of Clinical Otorhinolaryngology
基金
国家自然科学资助课题
