摘要
目的:观察钾通道与肺动脉平滑肌细胞(PASMC)增殖和凋亡关系中钙通道所起的作用。方法:将PASMC分为对照、钾通道阻断剂四乙胺TEA(开放剂比那地尔Pin)、钾通道阻断剂(开放剂)+钙通道阻断剂硝苯地平Nif(开放剂BayK8644)组,采用非核素增殖试剂盒、3HTdR掺入测定细胞增殖。将PASMC分为对照、钾通道开放剂、钾通道开放剂+钙通道开放剂组,采用细胞凋亡检测试剂盒测定细胞凋亡。结果:与对照组相比,10～20mmol/L的钾通道阻断剂TEA显著增加PASMC细胞数(P<0.01),15～30mmol/LTEA显著增加DNA合成(P<0.05,P<0.01)。TEA+5μmol/LNif组中只有20mmol/LTEA使DNA合成增加(P<0.05),其余浓度组与对照组均无统计学差异。TEA+Nif组与TEA组相比,TEA浓度在10～25mmol/L时2组细胞数相差显著(P<0.01),15～30mmol/LTEA时2组DNA合成相差显著(P<0.05,P<0.01)。与对照组相比,25～500μmol/LPin显著减少PASMC细胞数和DNA合成(P<0.01)。Pin+1μmol/LBay组与Pin组相比,当Pin浓度为25～500μmol/L时2组PASMC细胞数相差显著(P<0.01),当Pin浓度在50～500μmol/L时DNA合成相差显著(P<0.01)。Pin+Bay组与对照组相比,50～500μmol/L的Pin仍能显著减少PASMC细胞数(P<0.01),25～500μmol/L的Pin显著减少DNA合成(P<0.01)。与对照组相比。
Objective:To observe the role of calcium channel in the relationship between potassium channel and proliferation and apoptosis of pulmonary artery smooth muscle cells (PASMC).Methods: To detect cell proliferation, PASMC were divided into control, potassium channel blocker TEA (opener Pin), and potassium channel blocker (opener)+calcium channel blocker Nif (opener Bay K8644) groups, where cell proliferation was observed with a non-isotope proliferation kit and 3H-TdR incorporation. To detect cell apoptosis, PASMC were divided into control, potassium channel opener, potassium channel opener+calcium channel opener groups, where cell apoptosis was observed with a cell apoptosis test kit. Results: TEA significantly increased the cell number at 10-20 mmol/L (P<0.01) and DNA synthesis at 15-30 mmol/L (P<0.05,P<0.01) compared with the control group. There were no statistical differences between the groups of different concentrations of TEA+5 μmol/L Nif and the control group, except that TEA at 20 mmol/L significantly increased DNA synthesis (P<0.05). There were significant differences between TEA+Nif and TEA group only in cell number at 10-25 mmol/L (P<0.01) and DNA synthesis at 15-30 mmol/L(P<0.05,P<0.01). Pin at 25-500 mmol/L significantly decreased the cell number and DNA synthesis of PASMC compared with the control group (P<0.01). There were significant differences between Pin+1 μmol/L Bay group and the Pin group in cell number(Pin at 25-500 mmol/L) and DNA synthesis (Pin at 50-500 mmol/L) (P<0.01). In Pin+Bay group, Pin at 50-500 μmol/L significantly decreased the cell number and DNA synthesis at 25-500 mmol/L compared with the control group(P<0.01). Pin at 50-500 μmol/L significantly increased the apoptosis rate of PASMC compared with the control group (P<0.05, P<0.01). There were no significant differences in apoptosis rate between Pin+1 μmol/L Bay group and Pin group. Conclusion: Calcium channel plays an important role in the relationship between potassium channel and proliferation of PASMC, though has no ob
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第6期632-635,共4页
Academic Journal of Second Military Medical University
基金
上海市科委重点科研基金(034119828).
关键词
钙通道
钾通道
肺动脉
肌细胞
平滑肌
增殖
凋亡
calcium channels
potassium channels
pulmonary artery
myocytes,smooth muscle
proliferation
apoptosis
