摘要
作者建立改良的测定人血清中普罗帕酮反相ＨＰＬＣ法，固定相为ＬｉｃｈｒｏｓｏｒｂＲＰ－１８，流动相为甲醇：醋酸钠缓冲液：二乙胺（７４．５：２５：０．５ｖ／ｖ），流速为１ｍｌ／ｍｉｎ，以安定为内标，紫外检测波长２５０ｎｍ。普罗帕酮相对回收率９６．２５％～１０４．００％。日内和日间变异系数３．６％～７．８％，最低检测限为５ｎｇ，血浆中最低检测浓度４０ｎｇ／ｍｌ，在５０～３２００ｎｇ／ｍｌ浓度范围内线性良好，相关系数ｒ＝０．９９９８。用本方法测定１６名老年心律失常患者口服不同剂量普罗帕酮血浓，对老年患者服用普罗帕酮剂量─血浓─临床症状之间的关系作了观察。
n Improved quantitative method was reported for the determination of propafenone (PPF) in human plasma by reversed phase HPLC. The stationary phase was Lichrosob RP-18.The mobile phase was methanol-sodium acetate buffer-diethylamine(74.5:25 :0.5v/v),the flow rate was 1ml/min. Diazepam was used as internal standard. The detection was carried out atUV250nm. The relative recovery was 96.3～104.0%, The RSD of within-day or beween-day was 3.6～.8%. The detec-tion limit of PPF was 5ng or 40ng/ml in plasma,The method was linear within the range of 50 to 3200ng/ml and the correla-tion coefficient was 0.9998.The method was used for 16 elder patients with cardiac arrhythmias who were given orally withdifferent dosage of PPF. The relation of dosage-blood concentration-clinical symptom was observed.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1994年第3期99-101,共3页
Chinese Journal of Hospital Pharmacy
关键词
高效液相色谱
普罗帕酮
血药浓度
HPLC,propafenone,diazepam,elder patients with cardiac arrhythmias,plasma concentration
