摘要
用细胞生物学和SDS-PAGE电泳方法,研究抗阿霉素(Dox)CHO细胞系(RCl)的特性。用间接免疫荧光法,未测到RCl超表达P-糖蛋白。RCl降低Dox的摄入,增加膜的流动性,Dox在CHO中主要分布在细胞核,在RCl中则分布在细胞质和细胞核。RCl中有一种分子质量为30—40 kDa的核蛋白的表达被抑制,而在敏感CHO中其表达正常。
Some characteristics of doxoru-bicin-resistant CHO cell line (RCI) were studied by means of cell biological methods and SDS-PAGE electrophoresis. The resistance factor was 16. 5-fold , and RCI revealed cross-resistances to colchicine, actinomycin and har-ringtonine. By indirect immunofluorescence assay, P-glycoprotein was not detected. Compared with CHO, the doxorubicin (Dox) uptake and accumulation of RCI decreased, but the membrane fluidity of RCI increased. The reduction in drug accumulation was correlated with increase in membrane fluidity. Dox was mainly distributed in the cell nucleus of CHO, but in both cytoplasm and nucleus of RCI. This suggested that Dox was transported more slowly in RC1 cytoplasm than in CHO cytoplasm, resulting in less Dox entrance into the cell nucleus of RC1 than into that of CHO. We also found that a 30 - 40 kDa nuclear protein which was expressed normally in CHO disappeared in RC1.
出处
《中国药理学报》
CSCD
1994年第2期189-192,共4页
Acta Pharmacologica Sinica
基金
Project supported by the National Natural Science Foundation of China
№ 39270785
Doctor's Foundation of the Nationat Educationat Committee
№ 9202702.
关键词
阿霉素
膜流动性
抗药性
培养细胞
doxorubicin
glycoproteins
membrane fluidity
drug resistance
cultured cells, CHO cells
