摘要
应用异型双功能连接剂ＳＰＤＰ将抗人肺腺癌单克隆抗体ＣＭＵ１５Ａ与天花粉蛋白（ＴＣＳ）交联，制备出新型免疫毒素。应用的ＭＴＴ法检测其对人肺腺癌细胞系ＡＧＺＹ—８３Ａ的选择性杀伤活性。结果表明，ＣＭＵ１５Ａ—ＴＣＳ结合物具有高效靶向杀伤肺腺癌细胞能力，优于游离ＴＣＳ及单抗与ＴＣＳ混合物；与对非靶细胞的细胞毒作用相比较差异非常显著。提示该结合物是一种很有价值的免疫治疗剂，为今后的实验和临床应用提供了资料。
he immunotoxins were prepared by conjugating trichosanthin(TCS)with monoclonal antibodyCMU 15A.Study in vitro indicated that CMU 15A-TCS conjugate retained substantially the original im-munological activity of the CMU15A and showed stronger selective cytotoxicity against human lung ade-nocarcinoma cell line AGZY-83A(IC50 0.91 μg/ml)than free TCS(IC50 82μg/ml)and mixtures ofCMU 15A and TCS(IC50 60μg/ml),The difference was significant in comparison with cytotoxicity a-gainst nontarget cell line(Hela).The result suggested that CMU15A-TCS conjugate was an effective immunotoxin.
出处
《中国医科大学学报》
CSCD
1994年第4期335-338,共4页
Journal of China Medical University
关键词
免疫毒素
单克隆抗体
天花粉蛋白
癌
肺肿瘤
immunotoxin
antibody toxin conjugate
monoclonal antibody
trichosanthin
lung cancer
