摘要
目的 通过体外抗病毒实验研究芪黄冲剂在无明显细胞毒性浓度下对2 .2 .15细胞分泌HBsAg、HBeAg颗粒的影响,以验证芪黄冲剂对病毒复制的影响。方法 用MTT法测定芪黄冲剂在不同浓度下的细胞毒性,ELISA法检测芪黄冲剂及其拆方在不同浓度下在9～12d及第13天对共同培养的2 .2 .15细胞分泌HBsAg、HBeAg颗粒的影响。结果 芪黄冲剂1～4号在各浓度下均无明显细胞毒性,芪黄冲剂对2 .2 .15细胞分泌HBsAg、HBeAg颗粒有明显的抑制作用。结论 芪黄冲剂和其拆方组份(1～4组份)对HBV -DNA转染肿瘤细胞株(2 .2 .15细胞)无明显和直接的细胞毒性作用。芪黄冲剂可抑制2 .2 .15细胞分泌HBsAg、HBeAg ,具有良好的抗乙型肝炎病毒的作用。
Objective It is to study the effect of Qihuang granula on anti-virus of the 2.2.15 cells in vitro through measuring the quantity of HBsAg and HBeAg. Methods The cell toxicity of Qihuang granula under the different concentration was determined with MTT method, and the effect of Qihuang granula on HBsAg and HBeAg secreted by the 2.2.15 cell under the different concentration from the ninth day to the twelfth day and at the thirteenth day with ELISA method. Results Qihuang granula from No.1 to No.4 all had no obvious cell toxicity. Qihuang granula had obvious restrain effect on HBsAg and HBeAg secreted by the 2.2.15 cells. Conclusion Qihuang granula from No.1 to No.4 has no obvious and immediate cell toxicity effect on the 2.2.15 cells. Qihuang granula can restrain the 2.2.15 cells secreting HBsAg and HBeAg, and it has better effect of restraining HBV.
出处
《现代中西医结合杂志》
CAS
2005年第9期1137-1140,共4页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
上海市科委生物医药办公室资助课题 (99431 92 2 5 -8)
关键词
芪黄冲剂
体外
抗病毒
药效学研究
中药
chronic hepatitis B
Qihuang granula
HBsAg
HBeAg
HBV-DNA
2.2.15 cells
