摘要
目的观察哮喘大鼠肺泡Ⅱ型上皮细胞(AT_Ⅱ)损伤情况并探讨其可能机制。方法用卵白蛋白(OVA)制备Wistar大鼠哮喘模型,分别测定支气管肺泡灌洗液(BALF)中一氧化氮(NO)、肿瘤坏死因子_α(TNF_α)浓度并检测AT_Ⅱ细胞的凋亡率和坏死率,将AT_Ⅱ细胞凋亡和坏死率之和(总损伤率)与NO和TNF_α浓度进行相关分析。结果哮喘组AT_Ⅱ细胞的凋亡率和坏死率[(11.55±2.82)%和(10.69±3.99)%]分别显著高于正常对照组[(2.81±2.22)%和(1.84±0.95)%],P均<0.01;BALF中的TNF_α和NO[(93.23±19.84)pg/ml和(7.36±0.63)μmol/L]也显著高于正常对照组[(26.97±5.85)pg/ml和(3.99±0.42)μmol/L],P均<0.01;BALF中TNF_α和NO浓度分别与AT_Ⅱ细胞总损伤率呈显著正相关(P均<0.01)。结论哮喘大鼠AT_Ⅱ细胞损伤明显,而TNF_α及NO的生成和释放增加可能是导致AT_Ⅱ细胞损伤增加的重要原因。
Objective To investigate the injury of typeⅡepithelial cells(AT-Ⅱ)in rats with asthma and elucidate its mechanism.Methods Asthma model of Wister rat was established with ovalbumin(OVA)challenges.After the last challenge,these animals were sacrificed and bronchoveolar lavage fluid(BALF)was gained.The levels of nitrogen monoxide(NO)and tumor necrosis factor- alpha(TNF-α)in BALF were measured with nitrate reductase technique and sandwich ELISA respectively.After being isolated and purified,AT-Ⅱcells were detected for apoptosis and necrosis rates with flow cytometry(FCM).Correlation analysis between the total injury(including apoptosis and necrosis)rates of AT-Ⅱcells and the level of NO or TNF-αin BALF was measured respectively.Results Injury rates of AT-Ⅱcells in asthma group were significantly higher than those in control group(11.55%±2.82%vs2.81%±2.22%,P<0.01;10.69%±3.99%vs1.84%±0.95%,P<0.01).The levels of TNF-αand NO in control group were significantly lower than those in asthma group(26.97±5.85pg/ml vs93.23±19.84pg/ml,P<0.01;3.99±0.42vs7.36±0.63μmol/L,P<0.01)respectively.There was a positive correlation between the total injury rats of AT-Ⅱcells and the level of TNF-α(P<0.01)or NO(P<0.01).Conclusions Overproduction and release of TNF-αand NO maybe an important cause of AT-Ⅱcell injury in rats with asthma.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2005年第1期36-39,共4页
Journal of Clinical Pediatrics
关键词
哮喘
肺泡Ⅱ型上皮细胞
损伤
肿瘤坏死因子-Α
一氧化氮
asthma alveolar typeⅡepithelial cell injury tumor necrosis factor-alpha nitrogen monoxide
