摘要
目的 探讨红霉素是否对大鼠过敏性气道炎症具有保护作用以及是否通过抑制转录因子NF-κB的活性来达到抗炎效果。方法 用鸡卵清白蛋白 (OVA)腹腔注射一次致敏SD雄性大鼠,同时佐以氢氧化铝和百日咳杆菌, 2周后雾化吸入 1%的OVA进行激发,连续激发 7天,成功制作大鼠过敏性气道炎症模型。治疗干预组动物OVA激发的同时口服红霉素 [180mg/(kg·d) ]。最后一次激发后 24h处死大鼠,留取标本进行检测。用支气管肺泡灌洗液 (BALF)和肺组织病理分析的方法检测肺组织炎症;用免疫组织化学的方法检测核因子 kappaB(nuclearfactorkappaB, NF-κB)亚单位p65在细胞核内的表达;用凝胶电泳迁移改变分析的方法检测肺组织NF κB的结合活性。结果 肺组织病理切片显示红霉素治疗干预组肺组织炎症细胞浸润的范围较模型组明显减小。红霉素干预组BALF中白细胞总数为 (31±22)×108 /L,模型组为 (66±28)×108 /L(P<0.01 )。红霉素干预组主支气管黏膜下层单位面积内细胞核表达NF κB阳性的细胞数为 (1.4±0.4)×103 个 /mm2,模型组为(2.6±0.6)×103 个 /mm2 (P<0.01)。红霉素干预组动物肺组织转录因子NF-κB结合活性(32±14)低于模型组(46±17 ) (P<0.05 )。结论 红霉素干预组BALF中白细胞总数较模型组降低,主支气管黏膜下层单?
Objective To investigate whether erythromycin exerts anti-inflammatory effect on allergic airway inflammation and whether erythromycin modulate allergic airway inflammation by inhibiting nuclear factor kappa B (NF-κB) activation. Methods Ovalbumin (OVA) together with aluminum hydroxide and Bordetella Pertussis was injected intraperitoneally to immunize SD rats and two weeks later 1% OVA was inhaled to challenge them for consecutive 7 days to mimic allergic airway inflammation. In treatment group, erythromycin was given orally (180 mg/kg·d) during the course of allergen exposure. WBC counts in bronchoalveolar lavage fluid (BALF) and lung specimen analysis were used to describe lung tissue inflammation. The expression of NF-κB subunit p65 in cell nucleus of lung tissue was measured by immunohistochemistry and NF-κB binding activation in lung tissue by electrophoresis mobility shift assay (EMSA). Results Lung tissue specimen analysis indicated that the severity of allergic inflammation was reduced in treatment group. The number of total WBC in BALF (×10~8/L)(31±22)was lower than that in model group animals (66±28),P<0.01. The number (×10~3/mm^2) of cells with nuclear staining of NF-кB per square millimeter of submucosal region around large bronchus(1.4±0.4) was lower than that in model group animals (2.6±0.6),P<0.01. NF-кB binding activity (32±14)was lower than that of model group(46±17),P<0.05. Conclusion Erythromycin had an obvious protective role in allergic airway inflammation. Erythromycin inhibited NF-κB transcriptional activity to exert anti-inflammatory effect.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2005年第3期196-198,共3页
Chinese Journal of Pediatrics
