摘要
目的研究小分子热休克蛋白基因(sHsps)在小鼠胚胎前肢正常和异常发育过程中的表达情况。方法在GD10(查到阴栓之日为GD0)经口一次分别给予实验组孕鼠80mg/kg的视黄酸,对照组孕鼠给予等体积的植物油,并分别于GD11～GD18取两组胎鼠的前肢,利用RT PCR方法半定量检测15种Hsps的表达丰度。结果正常肢除Hspb4在GD12才开始表达外,其余在GD11～GD18均有表达;Hsp10、Hsp20、Hspb2、Hspb3表达丰度基本恒定;Hsp22、Hsp30随胚龄的增加而表达增高;Hsp25起初表达较高,以后下降并恒定在较低的水平;Hspb4在GD15、Hspb9在GD11和GD14出现高峰。多数基因在GD11～GD18的表达丰度实验组明显高于对照组。某些基因在正常和异常肢发育过程中表达明显不同,如Hsp25在实验肢和对照肢除表达趋势不同外,GD11～GD12对照肢表达丰度高于实验肢,而GD14～GD18则实验肢的表达丰度高于对照肢;实验肢Hspb7在GD11～GD12不表达,GD13开始表达,GD15后逐渐下降;实验肢Hspb10仅在GD12～GD16表达,其余胚龄不表达。结论不同Hsps的表达特点有所不同,多数Hsps在短肢异常发育中表达比正常肢明显升高,部分Hsps与正常肢发育表达模式明显不同。
Objective:To study the expression of small heat shock proteins genes (sHsps) in normal forelimbs and forelimb malformations during mouse embryogenesis.Methods: At gestational day (GD) 10,mice of the treatment and the control groups were administered with 80 mg/kg retinoic acid and the same volume of vegetable oil,respectively.The embryonic forelimbs were harvested in GD11-18.The relative abundance of 15 Hsps of all samples was measured by reverse transcript polymerase chain reaction (RT-PCR).Results: In the normal limbs,Hspb4 expressed from GD12,and all other 14 Hsps expressed in GD11-18.Also in the normal limbs,the expressional abundance of Hsp10,Hsp20, Hspb2 and Hspb3 was stable,and that of Hsp22,Hsp30 was increased with the aging of embryos.Hsp25 was highly expressed initially,and then descended and remained at a low level in the normal limbs.The expressional peak of Hspb4 was at GD15 in the normal limbs,and that of Hspb9 was at GD11 and GD14.The expressional abundance of most of the genes in the treatment group was higher than that of the control group in GD11-18.The expression of some genes was obviously different during the development of the normal and the abnormal limbs.For example,the expressional tendency of Hsp25 of the abnormal limbs and the normal limbs was different; the expressional abundance of the normal limbs was higher than that of the abnormal limbs in GD11-12,but lower than that of the abnormal limbs in GD14-18.The expression of Hspb7 started from GD13 in the abnormal limbs,and descended after GD15.The expression of Hspb10 in the abnormal limbs was detected in GD12-16 only.Conclusion: Different Hsps have different expressional characteristics.The expression abundance of most genes in the abnormal limbs is higher than that in the normal limbs.The expressional patterns of some genes in the abnormal limbs are obviously different from those of the normal limbs.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第5期509-514,共6页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30070662).
关键词
热休克蛋白基因
视黄酸
胚胎
肢
发育
heat shock protein gene
retinoic acid
embryo
limb
development
