摘要
本文将自动细胞分析技术引入抗早孕药物作用机理的研究,发现正常早孕妇女服用双炔失碳酯(AF_(53))或/和三苯氧胺(TMX)后,其胎盘绒毛滋养层细胞的DNA倍体水平无变化,用AP_(53)加TMX与各药单独应用相比,G_0+G_1期百分数明显为低(P<0.01),G_2+M期明显为高(P<0.01)。反映两药合用时,引起G_2M期细胞积聚,提示两药有协同作用(P(交至)≤0.01),以致细胞周期运转受阻。
Flow—cytometry was introduced in the mechanism study of early pregnancy—terminating drugs. It was found that DNA content in human placental chorio—trophoblastic cell had not changed at ploidy level in normal early pregnant women taking anordrin (AF_(53)) and /or tamoxifen (TMX). The percentage of cells in G_0+G_1 phase in the group of combined administration of AF53 and TMX was significantly lower(P<0.01),whereas that in G_2+M phase,it was much higher(P<0.01) than that in the group of single use of the above drugs. It revealed that the cells had accumulated in the G2 and M phase when the two drugs are used together,which suggested a synergetic action of the two drugs,resulting in a blocking of cell cycle.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
1994年第1期29-30,共2页
Chinese Journal of Practical Gynecology and Obstetrics
关键词
双炔失碳酯
三苯氧胺
滋养层细胞
Cell-cycle kinetics Anordrin Tamoxifen Human placental chorio-trophoblastic cell
