摘要
采用集成铜微电极的聚甲基丙烯酸甲酯(PMMA)电泳芯片,实现对氨基酸的分离与检测。考察了制备的铜微电极对氨基酸的催化活性以及检测电位和分离场强对分离效果的影响。在优化条件( 30mmol/LNaOH缓冲溶液中,检测电位为0. 7V(vs. Ag/AgCl),进样与分离电场强度均为50V/cm)下,用3cm长的通道实现了精氨酸(Arg)与亮氨酸(Leu)的分离。
The direct electrochemical detection of amino acids was accomplished on polymethyl. methacrylate (PMMA) capillary electrophoresis chip integrated with copper microelectrode. Factors influencing the performance, including the detection potential, separation field strength were studied. Under optimized detection conditions, i. e. 30 mmol/L NaOH buffer, 0.7 V detection potential and 50 V/cm separation field strength, the arginine and leucine were separated fast and well through a channel of 3 cm.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2005年第5期591-594,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No. 20299030
No. 20335040)
国家重大基础研究发展项目 (No. 2001CB5102
No. 2002CB513110 10 )资助项目
