摘要
用透射电镜观察了青海高原藏羊和牦牛源原头蚴接种小鼠后继发性细粒棘球蚴的超微结构。二源继发性棘球蚴囊壁结构相同，由角质层和生发膜构成。角质层由纤维物质和不规则形颗粒形成层状结构，但近生发膜处藏羊源的结构致密，牦牛源的则较为疏松。生发膜可分为皮层区和细胞区。皮层区外缘的微毛藏羊源多而短粗，牦牛源的则呈细长，与各自原发性棘球蚴相同，提示微毛的形态结构稳定，不随宿主不同而改变；牦牛源皮层区基部可见线粒体和大量糖原，提示该区有活跃的生理生化代谢功能。细胞区由皮层细胞和其它成分构成，皮层细胞内细胞器丰富，线粒体形态藏羊源的与原发性相同，牦牛源的则与原发性的存在差异，提示牦牛源棘球蚴线粒体的形态结构不稳定，易受不同宿主的影响。皮层细胞胞质的近囊腔处存在巨大的囊泡，囊壁多为单层。内缘附有线粒体或异染色质样物质，可能与后期子囊的形成有关。本文结果提示该地区可能存在不同的细粒棘球绦虫虫株。
The ultrastructural observation on the secondary echinococcus granulosus cysts in mice infected with protoscoleces from Tibetan sheep and yak origins in Qinghai plateau were carried out by transmission electron mocroscopy during 1991～1992. The structure of cysts wall of secondary echinococcus granulosus cysts in mice was similar between Tibetan sheep and yak origins ,and was comprised of the laminar layer and the germinal membrane.The laminar layer was layer-shape structure and was comprised of reticulae fibre and irregular electron-dense granular,but the structure was dense in Tibetan sheep original sample and was scatter in yak origin-al sample near germinal membrane. The germinal membrane were divided into tegument and cyton.The microtriches located in outer edge of tegument was short and thin in Tibetan sheep origin sample and was long and fine in yak origin sample,and similar to that of primary echinococcus granulosus cysts,It suggested that the structure and morphology of microtriches be stable,and glycogen mass could be seen in the basal part of tegument of yak origin sample,it suggested that the tegument have active physiological and biochemical functions.The tegumental cells had a great deal of organella,the morphology and structure of mitochondria were similar between primary and secondary echinococcus granulosus cysts in sheep origin sample,but those were different in yak(rigin sample.It suggested that the morphology and structure of mitochondria be not stable and were multi-change in different hosts.The massive vacuoles in which most of walls was single layer,and mitochondria attached to inner edge were observed,these were probably relative to form daughter cysts through development.The results of this paper suggested that the different strains of echinococcus granulosus should probably exist in Qinghai Plateau.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1994年第2期34-37,共4页
Chinese Journal of Zoonoses
关键词
原头蚴
继发性棘球蚴
超微结构
Tibetan sheep
Yak
protoscoleces
Secondary echinococcus granulosus cysts
Ultrastructure.
