摘要
目的探讨新疆锡伯族、哈萨克族和维吾尔族群体D7S820,D13S317和D16S5393个STR位点的基因型及等位基因频率的分布。方法随机抽取上述民族无关个体静脉血抗凝冻存,提取DNA并用复合PCR技术,同时扩增上述3个STR位点,PAGE垂直电泳后银染。结果3个少数民族群体上述3个STR位点均符合Hardy-Weinberg平衡定律。通过种族间比较,锡伯族与哈萨克族除D7S820位点无显著差异外,其他位点新疆锡伯族与哈萨克族和维吾尔族均有明显差异。结论3个STR位点的综合检验可用于群体遗传学研究和法医学应用。
Objective To get genotype and allele frequency distributions of D7S820, D13S317 and D16S539 loci in Xibo,Kazak and Uygur population in XinJiang. [WT10.HZ]Methods[WT] Blood specimens were colleted from unrelated Xibo, Kazak and Uygur individual in Xinjiang. The DNA samples were extracted and amplified by PCR. The PCR products were isolated by PAGE and were stained by silver nitrate. [WT10.HZ]Results[WT] All the 3 loci (D7S820, D13S317, D16S539) of Xibo, Kazak and Uygur population met Hardy-Weinberg equilibrium. There are significant difference between Xibo and Uygur population at three STR loci, and also between Xibo and Kazak at two STR loci, but there is no significant difference between Xibo and Kazak at D7S820. [WT10.HZ]Conclusions[WT] The three STRs were suitable for forensic identification and population genetic study as genetic markers.
出处
《基础医学与临床》
CSCD
北大核心
2005年第4期349-353,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(39670399)
