摘要
目的:检测人脊椎骨中是否存在褪黑素受体mRNA的表达,并探讨其意义。方法:取手术切除的人脊椎松质骨,抽提总RNA。用逆转录酶-聚合酶链反应(RT-PCR)方法检测褪黑素受体MT1、MT2mRNA的表达。结果:人脊椎骨总RNA以MT1和MT2两种受体引物进行的RT-PCR产物电泳呈阳性条带,其基因片段长度与MT1和MT2受体引物之间的核酸长度相当。结论:人脊椎骨中存在MT1、MT2两种褪黑素受体mRNA的表达。
Objective:To verify the expression of melatonin receptor mRNA in human vertebra,and study its significance.Method:Spongy bone of human vertebrae was collected to obtain total RNA and the mRNA of melatonin receptor was detected by reverse transcript-polymerase chain reaction(RT-PCR) method.Result:The electrophoretic results of RT-PCR products by MT1 and MT2 primer were all positive.Conclusion:There exists melatonin receptor gene in human vertebra.It suggests that melatonin may act on the spine directly and regulate its growth and development.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2005年第4期232-234,共3页
Chinese Journal of Spine and Spinal Cord
关键词
褪黑素
受体
脊椎
Melatonin
Receptor
Vertebra
