摘要
采用两步培养法培养新疆紫（Ａｒｎｅｂｉａｅｕｃｈｒｏｍａ（Ｒｏｙｌｅ）Ｊｏｈｎｓｔ）草细胞以生产紫草色素，建立了从２５０ｍｌ摇瓶放大到９Ｌ和２５Ｌ内循环气升式反应器的逐级放大培养程序。第一步细胞生长迅速，并有少量色素合成；第二步培养以生产色素为主，细胞生长明显减缓。研究了摇瓶培养时连续继代培养对细胞生长及色素合成的影响，以选择继代培养２～３代的细胞接入反应器中培养效果较好。９Ｌ反应器培养中通气率为０．４ｖｖｍ时可得到较高色素产量，对２５Ｌ反应器中混合及溶氧对细胞生长及色素合成的影响也进行了讨论。
two-stage culture method was developed to culture Arnebia euchroma(Royle) Johnst cells,and established a process of progressive scale-up culture from 250 ml shake flask to 9 L and 25 L airlift bioreactors with concentric draft tube. In the first stage,cells grew very fast,with the formation of a small amount of shikonin derivatives,In the second stage,cell growth was relatively slow,and a large amount of shikonin derivatives was preduced.The influence of successive subeulture in shake flask and different aeration rate of 9 L bioreactor on cell growth and shikonin derivatives formation was examined. The problem of mixing and oxygen supply on cell growth and product formation in 25 L bioreactor were also djscussed.
出处
《植物资源与环境》
CSCD
1994年第3期27-31,共5页
Journal of Plant Resources and Environment
基金
国家八.五攻关课题
关键词
新疆紫草
紫草色素
逐级放大培养
Arnebia euchroma (Royle)Johnst
shikonin derivatives: airlift bioreactor
progressive scale-up culture
