摘要
本文建立了拳卷地钱中黄酮类化合物的含量测定方法。采用改进的铝盐显色分光光度法测定拳卷地钱总黄酮含量;采用反相高效液相色谱法测定拳卷地钱黄酮类化合物中芹菜素、槲皮素和木犀草素的含量。分光光度法表明:总黄酮含量在1 6～16 μg/mL范围内,线性关系良好,加标回收率在97 6 1～10 1 5 9% ,相对标准偏差0 16 % ;反相高效液相色谱法表明平均加标回收率96 79～10 1 13% ,相对标准偏差0 6 6～1 5 2 %。本方法用于拳卷地钱中黄酮类化合物含量的测定准确可靠。
Methods for determining flavonoids from Marchantia convolute were established in this article.The contents of total flavones in Marchantia convolute were determined through developed visible spectrophotometry.The reversed phase high performance liquid chromatography was set up to determine the contents of flavonoids in Marchantia convolute.In visible spectrophotometry Beer's law was obeyed in range of 1 6～16 μg/mL and the recoveries were in the range of 97 61～101 59%.In HPLC apigenin,luteolin and quercetin were quantitated by calibration curve method at detection wavelength of 350 nm.The result shows that the average recovery is 96 79～101 13%,RSD is 0 66～1 52%.These methods were used to determine flavonoids in Marchantia convolute with satisfactory results.
出处
《天然产物研究与开发》
CAS
CSCD
2005年第2期186-189,194,共5页
Natural Product Research and Development
