摘要
百日草游离对肉细胞随着导管分子的分化，木质素含量逐渐增加；ＰＯⅠ（可溶性ＰＯ）、ＰＯⅡ（与细胞壁离子型结合的ＰＯ）和ＰＯⅢ（与细胞壁共价结合的ＰＯ）活性增加，并分别对底物愈创木酚、丁子香酚和咖啡酸（含阿魏酸）有较大的亲和力；抑制剂对ＰＯ活性抑制的动力学特性表明．间苯三酚为竞争性抑制剂，硫酸亚铁铵和二硫苏糖醇是非竞争性抑制剂。
Based on an experimental suystem of isolated Zinnia mesophyll cells, this paper describes the features of peroxidase (PO) acting in tracheary elements (TE) differentiation. During the differentiation of TE, lignin content gradually increased (Fig. 1);activities of PO Ⅰ(soluble PO), PO Ⅱ (PO ionicallybound with cell wall), PO Ⅲ (PO covalcntly bound with cell wall) increased, and the affinity of them to the substrates guaiacol, caryophlfene, caffeic acid and ferulic acid became higher (Fig. 2 and Table 2 ).The kineticcharacteristric of PO inhibition indicate that phloroglucinol is a competitive inhibitor,and ammonium ferrous sulfate and dithiothreitol are nonocompetitive ones (Fig.3 ).The relationship between PO activity, lignin synthesis and TE fornmation in TE-inducing culture and TE-inhibiting culture has been discussed.
基金
国家自然科学基金
关键词
过氧化物酶
叶肉
细胞
百日草
导管
lignin, peroxidase. inhibitor. tracheary element. differentiation. mesophyll cells. Zinnia elegans
