摘要
目的:研究半边旗提取物5F(以下简称5F)对HO-8910PM细胞内VEGF蛋白及ETS-1mRNA表达的影响,探讨其抗肿瘤侵袭转移的作用机制.方法:RT-PCR分析ETS-1mRNA的表达;Western blot分析VEGF蛋白的表达.结果:25～100 μmol/L 5F作用HO-8910PM细胞24 h后,明显下调ETS-1mRNA的表达;5F明显下调VEGF蛋白表达水平.结论:5F抗肿瘤侵袭转移能力与ETS-1mRNA和VEGF蛋白的表达有关.
Objective:To study the effect of 5F from Pteri semipinnata L on the expression of ETS-1mRNA and VEGF protein,and to investigate the antitumor mechanisms of 5F from Pteri semipinnata L.Methods:RT-PCR assay was used to assess the expression level of ETS-1 mRNA.The expression level of VEGF protein was assessed by Western blot analysis.Results:The expression level of of VEGF protein obviously decreased in HO-8910PM cells treated with 25～100 μmol/L 5F for 24 hours.5F significantly down-regulated the expression of ETS-1mRNA.Conclusion:The antitumor activity of 5F from Pteri semipinnata L is involved in the expression of ETS-1 mRNA and VEGF protein.
出处
《中药材》
CAS
CSCD
北大核心
2005年第4期307-309,共3页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金资助课题
No39870900
湛江市988科技兴湛计划项目
