摘要
目的: 建立大鼠异位全小肠移植的急性排斥反应动物模型,分析急性排斥反应过程中选择素的表达变化及其在排斥反应中的意义.方法: 选用近交系Wistar/A和F344 /N大鼠建立全小肠移植模型.实验共分4组,每组供受体各18只:第1组非手术对照组(F344 /N);第2组同基因移植组(F344 /N→F344 /N);第3组异基因移植组(F344 /N→Wistar/A);第4组异基因移植治疗组(F344 /N→Wistar/A+FK506).每组分别于术后第3, 5, 7日处死,在无菌条件下取移植肠行常规HE染色,观察组织学变化;免疫组织化学染色测组织中选择素的表达,同时检测移植肠中选择素mRNA表达的变化.结果: 异基因大鼠异位全小肠移植术后3, 5, 7d可出现典型的轻、中、重度排斥反应,而同基因组及异基因移植治疗组中未出现排斥反应. P 选择素在异基因移植术后早期显著升高,但随着排斥反应的加重,持续升高不明显,而E、L 选择素随着排斥反应的加重而持续升高,而同基因移植及治疗组选择素的升高不明显,且治疗组略低于同基因移植组.结论: 早期肠黏膜中P 选择素的表达对急性排斥反应的早期诊断及治疗有一定意义.
AIM: To establish the models of acute rejection in small bowel transplantation in rats,to analyze the changes of selectin expression on protein and gene level during small bowel allograft rejection in rats and to explore their significance in small bowel allograft rejection in rats.METHODS:Heterotopic small bowel transplantation was performed with inbred rat F344/N and Wistar.All recipients were divided into four groups: groupⅠof Wistar,groupⅡof F344/N→F344/N,group Ⅲof F344/N→Wistar/A,and group Ⅳof F344/N→Wistar/A+FK506.Six rats were put to death on post-operation day (POD)3,5 and 7 and the intestine was cut under sterile condition.All the samples were examined histologically and the expression of selectin was detected with immunohistochemistry technique.The intra- graft mRNA expression of selectin was determined.RESULTS:Histological examination showed that mild acute rejection occurred on POD 3 in group Ⅲ,moderate acute rejection on POD 5 and severe acute rejection on POD 7,while in groupⅡ,no acute rejection was found.The histological evidence of group Ⅳ indicated that FK506 could effectively control acute rejection.P-selectin,E-selectin and L-selectin were highly elevated in group Ⅲ on POD 3,significantly higher than those of other groups (P<0.01).E-selectin and L-selectin were elevated with the aggravation of the acute rejection while P-selectin was not significantly elevated.But the expression of the selectin was a bit elevated in group Ⅱ and group Ⅳ after the operation though the expression in group Ⅳ was lower than that in group Ⅱ.CONCLUSION: Early expression of P-selectin in small bowel transplantation rejection can predict the occurrence of acute rejection at an early stage.
出处
《第四军医大学学报》
北大核心
2005年第8期694-696,共3页
Journal of the Fourth Military Medical University
