摘要
从杭州郊区分离出一个ＴｕＭＶ强毒株系，利用分子克隆和Ｓａｎｇｅｒ双脱氢测序方法分析了其外壳蛋白基因的ＤＮＡ序列．该序列与已报道的其他４种ＴｕＭＶ分离物都具有较高的同源性，最高达９７．６％，最低达８９．５％，其氨基酸序列的同源性更高，最高达９７．９％，最低达９４．５％。本文分析了该序列中ＡＴ和ＧＣ含量，计算了４种核苷酸在有意义链中和在密码子不同位置上的出现频率，同时还分析了该病毒外壳蛋白氨基酸遗传密码的使用频率。
One turnip mosaic virus with strong infectivety was isolated in Hangzhou suburbs. The coat protein gene was cloned and sequenced by Sanger dideoxy method. It was samilar with four reported TuMV coat protein genes.The homolgy of nucleotides with each other was from89.5%to 97.6%and the homology of amino acid sequences from 94.5%to 97.9%, The percent-age of AT and GC,the nucleotide fraqueces and the position fraquences were caculated. The amino acid usage of coat protein was analysized.
基金
浙江省科委"八五"攻关课题
关键词
芜菁
花叶病毒
外壳蛋白
DNA
turnip mosaic virus
coat protein
DNA sequence
