摘要
在pH 9.2的H3BO3 NaOH缓冲溶液中,血清白蛋白使铅(Ⅱ) 抗坏血酸络合物在-0.51 V (vs.SCE)处的络合吸附波电流降低,电流降低值与加入的牛血清白蛋白(BSA)或人血清白蛋白(HSA)的浓度在一定范围内呈线性关系。BSA 在2 ~ 38μg/mL范围内,HSA在2~35μg/mL范围内呈线性关系,检出限均为1μg/mL。应用该法测定了人血清样品中蛋白质的含量,结果令人满意。
In this paper, the interaction of protein with Pb(Ⅱ)-ascorbic acid complex in pH 9.2 H_(3)BO_(3^-)NaOH solution has been studied by single sweep polarography. Adding BSA/HSA into the Pb(Ⅱ)-ascorbic acid solution decreases the current of the adsorptive peak of Pb(Ⅱ)-ascorbic acid complex. The △i_(p)′ is linearly proportional to the protein concentration from 2 μg/mL to 38 μg/mL for BSA and from (2 μg/mL) to 35 μg/mL for HSA. The detection limit is 1 μg/mL both for BSA and HSA. The method has been used for the determination of total proteins in human serum sample. The result is satisfactory.
出处
《分析科学学报》
CAS
CSCD
2005年第2期173-175,共3页
Journal of Analytical Science
基金
中南民族大学自然科学基金重点项目(YZZ03003)
