摘要
目的研究复方壳多糖真皮替代物对金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、白念珠菌的体外抑菌作用。方法制备复方壳多糖真皮替代物和复方胶原凝胶真皮,分别为实验组和对照组,各取50μL滴加在涂满细菌或真菌的平板,每种菌实验组和对照组样本各6个,孵育24h后观察抑菌环。结果复方壳多糖真皮替代物对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌具有抑菌作用,抑菌环均大于复方胶原凝胶真皮替代物,差异有统计学意义。两组对白念珠菌无抑菌作用。结论复方壳多糖真皮替代物是有体外抗细菌作用的组织工程产品。
Objective To evaluate antibacterial and antifungal effect of the composite chitosan artificial skin in vitro. Methods The standard strains were used in the experiment, including Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853) and Candida albicans (ATCC10231). Twelve agar plates were prepared for each standard strain, which were equally divided into the trial and the control groups. In the trial groups, 50 μL composite chitosan dermal substitute was added to each prepared agar plate, two samples for each plate. In the control groups, composite collagen-gelatin dermal substitute was used. After the plates were incubated at 35 ℃ for 18 ~ 24 h, the antibacterial or antifungal rings of every sample were measured. Results The composite chitosan dermal substitute showed the antibacterial effect on Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC25922), and Pseudomonas aeruginosa (ATCC27853) (P < 0.01, P < 0.05, and P < 0.001, respectively). Both composite chitosan dermal substitute and composite collagen-gelatin dermal substitute had no antifungal effect on Candida albicans (ATCC10231). Conclusion Composite chitosan artificial skin has the antibacterial effect in vitro, which may be used as a tissue-engineered substitute.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2005年第4期220-221,共2页
Chinese Journal of Dermatology
基金
国家863课题(2001AA216041
2003AA205022)
重庆市重点科技攻关项目(3897)
