摘要
研究表明，肿瘤坏死因子（ＴＮＨ）在５０～５０００Ｕ·ｍｌ－１范围内呈剂量依赖性地诱导牛脑微血管平滑肌细胞增殖，ＴＮＦ与该细胞培养２４ｈ时，即可明显刺激细胞增殖，４８ｈ时达最大刺激效应。欧芹素乙（ｉｍｐｅｒａｔｏｒｉｎ，Ｉｍｐ），异欧芹素乙（ｉｓｏ－ｉｍｐｅｒａｔｏｒｉｎ，Ｉｓｉ）在浓度为１０－６～１０－４ｍｏｌ·Ｌ－１时，均可剂量依赖性地拮抗ＴＮＦ诱导该细胞增殖。６－（α，α－二苯基乙酰哌嗪基苯基）－４，５－二氢－５－甲基－３（２Ｈ）哒嗪酮，６－（α－苯基乙酰哌嗪基苯基）－４，５－二氢－５－甲基－３（２Ｈ）哒嗪酮，则只在低浓度（１０－６ｍｏｌ·Ｌ－１）时拮抗ＴＮＦ诱导该细胞的增殖。
Effects
of tumor necrosis factor(TNF ) on the proliferation of bovine
cerebromic-rovascular smooth muscle cells(BCSMC) were investigated.At
concentrations from 5 0 to 5 0 0 0U· ml-1, TNF was shown to induce
proliferation of cultured BCSMC in a dose-dependent manner. After 24
h incubation of the cells,TNF significantly stimulated the
proliferation of BCSMC andreached maximal effects after 48 h
incubation , then the effects slightly decreased.At concentrations
from l 0-6 to l 0-4mol·L-1,both imperatorin ( Imp) and
iso-imperatorin(Isi)were found to antagonize the TNF induced
proliferation of BCSMC. Their maximal inhibitoryeffects were 42. 2
and 36.1%at l0-4 mol·L-1 respectively.6-(α,α
-diphenylacetylpiperazinly)pheny1-5- methyl-4,5-
dihydro-3(2H)-pyridazinone(DMDP ) and 6-(α
-phenylacetylpiperazinyl)phenyl- 5- methy-4,5-dihydro-3(2H)-
pyridazinone (PMDP) were also found to possess similar effectat lower
concentration(10-6mol·L-1),but no significant effect was observed
when the drugconcentration was higher.
出处
《药学学报》
CAS
CSCD
北大核心
1994年第2期86-89,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
关键词
肿瘤坏死因子
平滑肌
欧芹素乙
Tumor necrosis
factor
Smooth muscle
Imperatorin
Iso-imperatorin
Pyridazinone
derivatives
