摘要
目的 研究维生素E琥珀酸酯 (VES)对胃癌细胞的诱导分化作用。方法 5 ,10 μg/ml维生素E琥珀酸酯处理人胃癌SGE - 790 1细胞 ,分别采用噻唑蓝 (MTT)法检测细胞的生长 ;3 H -TdR掺入法测定DNA合成速率 ;流式细胞术检测细胞周期的分布 ;分光光度法检测分化标志酶的活性。结果 VES可使细胞形态学改变 ;抑制SGC -790 1细胞的生长 ,降低DNA的合成速率 ;使细胞出现G0 /G1期停滞 ;分化标志酶碱性磷酸酶 (ALP)、乳酸脱氢酶(LDH)活性降低。结论 VES可诱导SGC - 790
Objective To study differentiation-induction effect of vitamin E succinate(VES) on human gastric carcinoma cell line SGC-7901.Methods At the base of cell culture in vitro ,the effect of differentiation-inducing activity of VES at the dose of 5,10?μg/ml were measured by MTT method for cell growth rate;by 3H-TdR incorporation method for DNA synthetic speed;by flow cytometry for cell cycle distribution,by spectrophotometer for enzyme activity.Results After treated with VES,SGC-7901 cells were led to morphologic change,growth inhibition,DNA synthetic speed decrease,cell cycle arrest,and ALP,LDH activity suppressant.Conclusion VES can induce SGC-7901 human gastric cancer cells to undergo differentiation.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第11期1308-1309,共2页
Chinese Journal of Public Health
基金
国家自然科学基金资助 (39970 4 7)
