摘要
目的建立HPLC直接分离包甲素类似物对映体的方法。方法在正相条件下,使用CHIRALPAKAD分析柱,考察了流动相的组成、流速、柱温对对映体拆分的影响;应用不同体积比的正已烷、异丙醇、二乙胺为流动相,不同的流速,对4个包甲素类似物(Ⅰ、Ⅱ、Ⅲ、Ⅳ)外消旋体分别进行了手性分离。结果得到较佳拆分色谱条件,4个包甲素类似物都能实现对映异构体的基线分离。结论此方法适用于包甲素类似物的光学纯度分析。
Objective To develop a chiral separation method for the enantiomers of analogs of Baogongteng A by using the mobile phase constituted of n-hexane, 2-propanol and diethylamine with column CHIRALPAK AD. Methods The chromatographic conditions such as mobile phase, flow rate, temperature of chiral separation were investigated. Results Optimum chiral separation for racemates of four analogs of Baogongteng A ( Ⅰ , Ⅱ , Ⅲ ,Ⅳ ) was obtained. Conclusion The method is efficacious, convenient and reliable. It can be used for the analysis of optical purity of analogs of Baogongteng A.
出处
《上海第二医科大学学报》
CSCD
北大核心
2005年第3期229-231,共3页
Acta Universitatis Medicinalis Secondae Shanghai
基金
上海市科委重点医药研究基金(034319230)上海市教委学科建设专项基金(JY2001)资助项目.
