摘要
利用RAPD分子标记技术研究了我国喀斯特高海拔山区适宜玉米自交系的遗传多样性,用23条引物对我国喀斯特高海拔山区适宜玉米自交系进行遗传多样性研究和杂种优势群划分。从90个随机引物中筛选出23个多态性好的引物扩增材料,产生出201条多态带,多态性位点比率为81.6%,通过UPGMA聚类分析,将我国喀斯特高海拔山区玉米地方自交系划分为7个类群。
RAPD markers technique has been used to study the diversity of maize suitable inbred lines in Karst high elevation mountainous area. The screening of the 90 random primers allowed the selection of 23 polymorphic primer used for the analysis. A total of 201 polymorphic bands were produced from the 23 selected primers. Through clustering on RAPD markers by UPGMA(Unweighed Pair-Group Method Using Arithmetic Average), Mean polymorphic information content was 0.651. Clustering analysis was conducted basing on the genetic similarity coefficient. The maize suitable inbreds were classified into seven groups.
出处
《玉米科学》
CAS
CSCD
北大核心
2005年第1期10-14,共5页
Journal of Maize Sciences
基金
贵州省"十五"重大攻关项目[黔科合农社字2001(1104号)]
贵州大学校基金项目赞助
