摘要
目的观察葡萄籽多酚(GSPE)对糖尿病大鼠体内非酶糖基化反应的影响,评价其对糖尿病慢性并发症(DCC)的干预效果,并探讨其作用机制。方法Wistar大鼠腹腔注射链脲佐菌素(STZ)60mg/kg造成糖尿病模型,随机分为空白对照组、氨基胍(150mg·kg-1·d-1)治疗组、GSPE低、中、高3个剂量(50、150、450mg·kg-1·d-1)治疗组,每组12只,另12只设为正常对照组。常规喂养12周后取大鼠血清及脏器,比较各组肾重/体重比值,用荧光法比较各组肾皮质非酶糖基化终产物(AGEs),全自动生化仪检测血糖、总胆固醇(TC)和甘油三酯(TG)。结果与空白对照组比较,GSPE可以显著降低糖尿病大鼠肾重/体重比值(P<001),减少肾皮质AGEs(P<001),GSPE中、高剂量组与氨基胍治疗组作用相似;GSPE对血糖、TG无显著影响,仅GSPE中剂量组TC与正常对照组比较差异有显著性(P<001)。结论GSPE能够抑制体内非酶糖基化反应,是其防治DCC的主要作用机制之一。
Objective Observing anti-nonenzyme glycosylation effect of grape seed proanthocyanidin extract (GSPE) in diabetic rats, evaluating the effect of GSPE on preventing and curing diabetic chronic complications (DCC) and probing into its mechanism. Methods (1) Animals and treatments: 12 Wistar rats were randomly selected as normal control group; the others were reproduced into diabetic animal models by streptozotocin (60 mg/kg) abdominal cavity injection. The rats of blood sugar>16.7 mmol/L were selected out 72 hours later, and then were randomly divided into blank control group, aminoguanidin (AG) treatment group, and low, middle, high dose GSPE treatment groups. There were 12 rats in each group respectively. The normal and blank group were treated with tap water, the AG group was treated with AG (150 mg·kg^-1 ·d^-1 ), the other three groups were treated with GSPE (50、150、450 mg·kg^-1 ·d^-1 respectively). No insulin was used during the whole experiment. (2) Samples taking: All the rats were fasted at least 6 hours at the end of the experiment. Their blood samples were drawn after body weights (BW) were measured. Double kidneys of each rats were taken out, and the renal weight (RW) was measured. (3) Measurements:blood sugar, total cholesterol (TC), triglyceride (TG) were detected by Bayer DVI 1650 automatic machine. The advanced glycosylated end products (AGEs) of renal cortex were compared with fluorescent method in each group. (4) Statistical analysis: Results were expressed as means±SD. Student’s-t test and one-way-anova were used in order to evaluate the differences between each group by SPSS 10.0 software. Results Compared with the blank group, the RW/BW ratios were decreased markedly by AG and GSPE treatments(P<0.01) at 12~th weekend. The AGEs in renal cortex were decreased markedly by AG and GSPE treatments (P<0.01). The effects of middle, high dose GSPE were equal to AG (P>0.05). The blood sugar,TG did not change distinctly in the treatment groups(P>0.05) during the experiment. The TC of middle dose
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2005年第1期49-52,共4页
Chinese Journal of Geriatrics
基金
山东省医药卫生科技发展计划项目(HW067)
