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Sorghum属7个近似种的DNA微量提取方法比较 被引量:3

The comparison of several methods on DNA microextraction of seven related specics in Sorghum.
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摘要 本研究以Sorghum属中 7个近似种的种子为试验材料 ,采用CTAB、SDS、CTAB与SDS相结合及TaKaRaDNA提取试剂盒等 4种方法提取DNA ,并对不同提取方法所获取的DNA纯度、浓度及DNA提取率进行研究分析比较。结果表明 :采用Moller方法对其 7个近似种的基因组DNA的提取为最佳方法。 DNA in seven related species weeds of Sorghum was isolated to use four methods including CTAB、SDS、Moller and TaKaRa DNA extraction kit for GMO Detection. The purity,concentration and extraction ratios of DNA in the products obtained by different methods was tested. Results showed that Moller was the best method to extract the genomic DNA in weeds of the seven species.
出处 《植物检疫》 2005年第2期65-68,共4页 Plant Quarantine
基金 国家质量检验检疫监督总局 2 0 0 2年科技项目 计划编号 :IK2 0 0 2 0 5 9
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