摘要
本文测定的黑曲霉纤维素酶各组分均为糖蛋白，但含糖量和组成各不相同，含糖量分别为：β－葡萄糖苷酶１１．３％，内切β－葡聚糖酶１１．８％，β－葡聚糖纤维二糖水解酶ＣＢＨ—１７．２％、ＣＢＨ－Ⅱ５．８％。各酶组分的氨基酸组成有一定的差异，但也有一些共同之处，即都含有较多的酸性氨基酸（Ａｓｐ、Ｇｌｕ），碱性氨基酸（Ｈｉｓ、Ａｒｇ）含量较低。分析酶组分间的相似性时发现，β－葡聚精纤维二糖水解酶的ＣＢＨ－Ⅰ组分与内切β－葡聚糖酶在各种氨基酸的含量上较为相似，而同属于β－葡聚糖纤维二糖水解酶的两个组分ＣＢＨⅠ和ＣＢＨ—Ⅱ在氨基酸的含量上有一定的差异。
The cellulases purified from Aspergillus niger were all glycoprotein,the carbohydrate contents of β-glucosidase, End-β-glucanase and two components of cellobiohydrolaseCBH-Ⅰ, CBH-Ⅱ were 11.3g%, 11. 8%, 7. 2% and 5. 8%, respectively. Their amino acid compositions were different from one another, but all the four enzyme component contained more acidic amino acids(Asp, Gin), and less basic amiaoa acids (His, Arg. ) The Amino acid compositions of CBH-Ⅰ and End-β-glucanase were similar to each other,but that of CBH-Ⅰ and CBH-Ⅱ were quite different from each other.
出处
《微生物学杂志》
CAS
CSCD
1994年第1期6-8,共3页
Journal of Microbiology
关键词
黑曲霉
纤维素酶
化学成分
Aspergillus niger
cellulase amino acid composition glycoportein
