摘要
以谷氨酸生产菌天津短杆菌（Ｂｒｅｖｉｂａｃｔｅｒｉｕｍｔｉｅｎｔｓｉｎｅｎｓ）Ｔ６－１３为出发菌株，经菌体或原生质体紫外线（ＵＶ）、氯化锂、香豆素等多因子诱变和高温驯化，选育出琥珀酸、生物素营养缺陷型，耐高糖、耐高谷氨酸又不利用谷氨酸，且温度适应范围大（既可在常温３２－３８℃发酵，又可在高温３６－４２℃发酵）的突变株ＧＭＨ－９０８。以淀粉糖为碳源，生物素亚适量，摇瓶常温发酵产酸９５ｇ／Ｌ以上，高温发酵产酸７４．９ｇ／Ｌ，比出发菌株Ｔ６－１３分别提高２６．６７％和２２．９９％；工业生产常温发酵月平均产酸８５－８８ｇ／Ｌ，转化率５２－５４ｇ％，单罐最高产酸１０８ｇ／Ｌ，转化率５８％；高温发酵产酸达７６．５ｇ／Ｌ，转化率４９．５％。
The initial strain Brevibacterium Brentsinens T6-13 was mutagenized by multi factors such as ultraviolet, LiCl, coumarin on free cell or protoplast and high femperature taming. A double auxotroph (succinic acid-and; biotin-requirement)was obtained. This mutant GMH-908, which has become resistant to high concentration of carbonhydrate and L-glutami cacid, but can not use L-glutamic acid, has a wide temperature adaptation (fermenting both at normal temperature 32-38℃ and at high temperature 36-42℃). When starch hydrolysate was used as carbon sources and biotin was in submoderaie amount, the yield of L-glutamic acid reached 95g/L or more at normal temperature and 74.9g/L at high temperature in shaking flask,increasing by 26.67% and 22. 99% respectively as compared with the initial strain T6-13. The monthly ayerage production of L-glutarnic acid at normal temperature was 85-88g/L and the conversion rate of glucose to L-glutamic acid was 52-54% on industrial scale, while the maximal. production of 108g/L of L-glutamic acid and 58% of conversion rate were found in single-vessel fermentation; 75.69/L of L-glutamic acid and 49. 5% of conversion rate were yielded at high temperature.
出处
《微生物学杂志》
CAS
CSCD
1994年第2期17-22,共6页
Journal of Microbiology
关键词
谷氨酸
诱变育种
发酵
温度
细菌
L-glutamic acid
selection
fermentation
temperature
