摘要
检测６例活动期、６例缓解期系统性红斑狼疮（ＳＬＥ）患者和１０例正常人血清和外周血单个核细胞（ＦＢＭＣ）自发及经天然ＤＮＡ（ｎＤＮＡ）、重组白介素２（ｒＩＬ－２）和重组γ干扰素（ｒＩＦＮ－γ）锈导后分泌ＩｇＧ类抗双链ＤＮＡ（ｄｓＤＮＡ）特异性抗体水平，观察到活动期患者血清中含量和自发分泌量显著高于缓解期，后者又显著高于正常人，且体外细胞分泌水平与血清中含量密切相关（ｒ＝０．９８８２）。ｎＤＮＡ能特异地激发ＳＬＢ患者的ＰＢＭＸＣ分泌抗ｄｓＤＮＡ抗体，但对正常人无作用。ｒＩＬ－２和ｒＩＦＮ－γ皆能增强ＳＬＥ患者ＰＢＭＣ产生抗ｄｅＤＮＡ抗体。ｎＤＮＡ和ｒＩＬ－２联合应用时对活动期患者ＰＢＭＣ分泌抗ｄｓＤＮＡ抗体有协同作用。作者未发现重组白介素６（ｒＩＬ－６）对抗ｄｓＤＮＡ抗体分泌的影响。
Peripheral blood mononuclear cells(PBMC) from 6 patients with active and 6 with inactive SLE were cultured in vitro, and the amounts of specific anti-double stranded DNA(anti-dsDNA) antibodies of the IgG class Were determined in the the culture sapernatant before and after stimulation.Stimulation was effected by native DNA(nDNA) and cytokines(rIL-2,rIL-6,rIFN-γ).A group of 10 sex and age matched controls were also researched.It was shown that the antibody level in sera of actlve SLE patients and their PBMC culture supernatant before stimulation was significantly higher than that from subjects with inactive SLE.But the latter showed marked elevation in comparison with that from normal controls.The level of anti-dsDNA secretion in vitro showed close correlation with that in sera(r=0.9882).nDNA can specifically stimulate PBMC from SLE patients to secrete anti-dsDNA antibody but has no effect on normal lymphocytes.Both rIL2 and rIFN-γ are able to enhance anti-dsDNA antibody production by PBMC from active and inactive SLE patients.We also found that nDNA antigen can coact with rIL-2 to induce anti-dsDNA antibody secretion by PBMC in active SLE patients.We did not find any obvious effect of exogenous rIL-6 on anti-dsDNA antibody secretion.
出处
《上海医科大学学报》
CSCD
1994年第2期109-112,共4页
Journal of Fudan University(Medical Science)
基金
卫生部科研基金
关键词
红斑狼疮
抗体
DNA
重组
干扰素
systemic lupus erythematosus
anti-double stranded DNA anti body
native DNA
recombinant interleukin2
recombinant interferon-γ
