摘要
在建立高活力肿瘤浸润淋巴细胞（ＴＩＬ）分离培养方法的基础上，对８３例恶性实体瘤ＴＩＬ的细胞增殖动力学、部份ＴＩＬ表型与杀伤力等作了较为系统的研究。结果提示，本实验室所建立分离培养的ＴＩＬ增殖能力较强且稳定，有６５％的增殖倍数大于１０００倍；３Ｈ－ＴｄＲ掺入高峰在培养的第４５～７５天；对其自身的原代肿瘤细胞杀伤力可以维持至５６天以上；对５６份经ｒＩＬ２诱导的ＴＩＬ（培养时间１７±５．２天）表型分析：ＣＤ３８０±２１％，ＣＤ４３７±２１％，ＣＤ８４４±１８％，ＨＬＡＤＲ６９±２４％，与ｒＩＬ２诱导前比较，ＣＤ３、ＣＤ４、ＣＤ８和ＨＬＡＤＲ均增多，尤以ＣＤ３与ＣＤ８表型增多明显。
After setting up a new isolation and culture method for tumor-infiltrating lymphocytes (TILs),the proliferation,cytotoxicity and phenotype of TILs from 83 cases of solid tumor were studied. The results showed that 65% of TILs had a proliferation over 1000 folds;3H-TdR incorporation rate peaked at 45￣75 days ;cytotoxity against tumor cells could maintain 56. days. The phenotypes of TILs after induction by rIL2 were CD3 80±21 %,CD4 37 ±21 %,CD8 44±18% and HLA DR 69±24%,CD3 and CD8 were substantially higher than that before induction.
出处
《上海免疫学杂志》
CSCD
北大核心
1994年第5期257-260,共4页
Shanghai Journal of Immunology
关键词
肿瘤
浸润淋巴细胞
淋巴细胞表型
流式细胞仪
tumor-infiltrating lymphocyte (TIL) lymphocyte phenotypes and subsets flow cytometry (FACS) autologous tumor cells specific cytotoxicity
