摘要
我们选用美国国立卫生院重组DNA咨询委员会批准的LN系列小鼠缺陷型逆转录病毒LXSN作为载体,对肿瘤坏死因子基因转导TIL进行了研究和初步的临床应用,实验结果显示,最佳转导时间为TIL分离培养后9-25d,即对数生长期间,同时,MOI值影响着转导效率,MOI值越大,转导效率越高.PCR及L929细胞的检测均表明了TNF基因的成功插入.一例胃癌肝转移患者初步临床应用的结果表明,TIL的高度特异性及治疗途径的选择和治疗方案的适宜对于提高疗效,减少TNF毒副反应起了一定作用.
We selected defective retrovirus LXSN approved by the recombinant DNA Advisory Comittee, NIH, USA as a vector to study the transduction of human TIL with TNF gene, on the basis of which, we engaged in the clinical application. The results showed that the logarithmic growth phase was the optimal transducing time when the TIL were isolated and cultured from 9 to 25 days. It also showed that the transduction efficiency was correlated to the MOI value, the more MOI value, the more TIL being transduced. Successful gene inserted and expressing were confirmed by the polymerase chain reaction and the L929 cell test. The clinical trial of treatment for metastastic hepatic cancer suggested that the better therapeutic effectiveness and less side effect depend on the specificity of the TIL, the proper infusion pathway and the dose of the transduced TIL.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
1995年第1期11-15,共5页
Chinese Journal of Cancer Biotherapy
基金
本课题为国家"863"重点课题
