摘要
建立了一种快速、灵敏检测痕量展青霉素（ｐａｔｕｌｉｎ）和青霉酸（ｐｅｎｉｃｉｌｌｉｃａｃｉｄ）的新方法。对酞内酰胺苯甲酰氯（４（２－ｐｈｔｈａｌｉｎｕｄｙｌ）ｂｅｎｚｏｙｌｃｈｌｏｒｉｄｅ，简称ＰＩＢ－Ｃｌ］为柱前衍生试剂同展青霉素和青霉酸衍生反应，衍生物用ＯＤＳ柱分离，乙腈－水（４７：５３，Ｖ／Ｖ）作流动相，紫外检测器检测（λ＝３００ｎｍ）。以２倍信噪比计算最低检出限，展青霉素２．０ｐｍｏｌ，青霉酸１０ｐｍｏｌ。
new and sensitive method was developed for the determination of trace patulin and penicllic acid byHPLC using ultraviolet detection. The method involves the esterification of patulin and penicillic acid by useof a derivative reagent , 4-(2-phthalimidyl)benzoyl chloride. The esterified toxins were simultaneously sepa-rated on an ODS column with acetonitrile-water(47:53,V/V)under acidic condition as the mobile phase anddetected at UV 3 0Onm. The detection limits of patulin and penicillic ac id were 2.0 pmol and 10 pmolrespectively.
出处
《色谱》
CAS
CSCD
北大核心
1994年第2期87-89,共3页
Chinese Journal of Chromatography
关键词
高效液相色谱
展青霉素
青霉酸
high performance liquid chromatography,patulin,penicillic acid
