摘要
阐述把维生素Ｂ１２生产菌添加到培养水中培养褶皱臂尾轮虫ＢｒａｃｈｉｏｎｕｓＰｌｉｃａｔｉｌｉｓ的买验。共１８株细菌分离于轮虫培养池，其中，有一株产维生素Ｂ１２的假单胞杆菌ＴＰ４对轮虫的生长繁殖有明显的促进作用。把ＴＰ４菌株培养后，加入到２Ｌ的烧杯和５００Ｌ的水槽中培养泰国Ｓ型轮虫时．在９ｄ（天）和６ｄ（天）中，轮虫密度从１２４～１３９和２４２～２８８个体／ｍｌ增殖到４，４１７～５，５４０和１，０１７～１，２５４个体／ｍｌ，分别比对照组增加了４～６及２～３倍。
Tests of adding vitamin B12-producing bacteria were carried out for the eulturing of the rotifer Brachinous plicotilis (Thailand S strain). Among 18 bacterial strains isolated from rotifer culture tank, one strain of Pseudomonas, TP4, a vitamin B12 producer, appeared a significant complementary effect on rotifer growth. Cultures with the strain TP4 addition showed that the rotifer density increased from 124- 139 to 4,417- 4, 540 ind. /ml in 2 liter beakers and from 242 - 288 to 1, 017 - 1, 254 ind. /ml in 500 liter tanks, and the increased rates were 4- 6 and 2- 3 times higher than that of the controls during the culture periods of 9 and 6 days, respectively.
关键词
褶皱臂尾轮虫
维生素
生产菌
培养
Brachionus Plicatilis
vitamin B12-producing bacteria: rotifer culture
