摘要
应用蚕豆根尖细胞微核试验和小鼠骨髓细胞微核试验两种测试法研究了硒与铬对微核率的影响．实验结果表明，在本试验浓度与剂量范围内，硒与铬均有较强的致突变效应，与对照组相比，有显著性差异（ｐ＜０．０５；Ｐ＜０．０１；ｐ＜０．００１），并在一定浓度或剂量范围内，有良好的刘量效应关系；同时实验结果亦表明，硒能明显地降低重铬酸钾诱发的微核率，提示硒对重铬酸钾的诱变效应有抑制作用，但硒抗重铬酸钾的诱变效应并非硒的剂量或浓度越大，效果越好，从试验结果来看，只有在一定的剂量／浓度范围内以及适当的剂量／浓度配比下，才能显示最佳的抗诱变效应．亚硒酸钠具有二重性，当它同致突变物质共同作用时，能够起到抑制突变，维护正常基因传递的作用，而在其单独作用时，却有一定的致突变性，我们的实验结果证明了这一点，因此应用硒进行肿瘤化学预防研究时选择适当的剂量是致关重要的．
The effects 6f selenium and cadmium on micronucleus frequency were observed in a micronucleus test of root tip cells in Vivia Faba and a mouse bone-marrow micronucleus test. The results indicated that selenium and cadmium both have the mutagenic effects. The differences compared with controls were statistically significant at a p value of less than 0.05. 0.01. 0.001, respectively. Both of the chemicals have the clear doseeffect relationship in a certain range of doses or concentrations. The results obtained also show that selenium can apparently decreas the micronucleus frequency induced by pot4ssium dichromate. This demonstrated that selenium was effective in inactivating themutagenicity of potassium dichromate. However, the optimal antimutagenesis effect couldonly be observed in a suitable range and a suitable ratioi of dose / concentration. Sodium selenite has a double character. When it acted together with mutageon, it could inhibit the mutagenic effect and protect the normal gene transference. When it acted alone, it exhibited an unambigUous mutagenic effect.The present results also indicate that it is important to chose the proper dose at the time of using selenium for research and prevention of the mutagenic toxicity.
出处
《南京大学学报（自然科学版）》
CSCD
1994年第3期477-481,共5页
Journal of Nanjing University（Natural Science）
关键词
微核试验
抗诱变性
重铬酸钾
硒
micronucleus test, antimutagenesis, soldium selenite, potassium dichromate
