摘要
本文用纯化的早孕因子（ＥＰＦ）免疫ＢＡＬＢ／ｃ小鼠，通过鼠－鼠杂交瘤技术，成功地建立了２株（１Ｂ＿６、２Ｆ＿（１０））分泌ＥＰＦ单克隆抗体（ＭｃＡｂ）的杂交瘤细胞系。经检测，它们所分泌的抗体亚类均为ＩｇＧ＿１，杂交瘤染色体数目１００～１０６条，ＥＬＩＳＡ检测诱生腹水抗体效价为１：１．２８×１０￣５，连续培养生物学性状稳定。抗ＥＰＦ抗原的单克隆抗体的建立，为深入研究ＥＰＦ的生物学性质、特征提供了有力的工具。
In the present study,two hybridoma cell lines (1B_6,2F_(10))secreting monoclonal antibodies. The resultsshowed that the immunoglobulin subclass of McAbs 1B_6 2F_(10) were IgG1 and the number of chromosomes of 2 hy-dridoma cell lines (1B_6, 2F_(10))ranged from 100～106.The titer of the EPF McAbs in the ascites was 1:1.28×10￣5 byELISA, The MxAbs against human EPF may be useful tool for studing biologic properties and function of human EPF.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1994年第4期255-257,共3页
Immunological Journal
关键词
早孕因子
单克隆抗体
ELISA
Early pregnancy factor, ELISA, Monoclonal antibody
