摘要
用根癌农杆菌感染芥菜型油菜子叶外植体，培养２～３ｄ，然后转到含Ｋｍ１０～２０ｍｇ／Ｌ的选择培养基上筛选转化体，在选择培养基上获得的Ｋｍ抗性苗，进一步在含Ｋｍ３０～５０ｍｇ／Ｌ的选择培养基上筛选３～４月，然后诱导生根，再生成完整植株．ＤＮＡ分子杂交证明Ｂ．ｔ．基因已整合到油菜基因组中，转化频率为１％～２％．
In this paper, we report the transformation of rape (Brassica Juncea).The cotyledons with the petioles cut from exenic seedings were infected with Agrobacterium tumefaciens strain AST 367 (pBS21 ), and cultured on MS selective medium containing 10￣20 mg/L kanamycin and 4. 5 mg/L 6-BA To select transformants. The transformed shoots were further subcultured on the selective medium containing 30￣50 mg/L kanamycin for 3￣4 months,and then rooted on MS medium containing 0. 5 mg/L IBA and 20 mg/L Km. The transformation frequency was about 1%￣2%. Southern blot hybridization showed that some of the plants gave positive signal with insecticidal gene(B. t. gene)probe. The Plants were transplanted to soil in pots and developed to mature. seeds were obtained from self-pollinated transgenic plants.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
1994年第4期528-542,共15页
Journal of Central China Normal University：Natural Sciences
基金
国家"863"计划资助
关键词
芥菜型油菜
基因
遗传转化
油菜
rape (Brassica juncea )
B. t. gene
transformation
