摘要
用照射小鼠的骨髓造血干细胞(CFU-S)外源性脾结节形成法,评价人基因工程重组SOD(rhSOD),聚乙二醇(PEG)修饰的长半衰期重组SOD(PEG-SOD)对CFU-S的辐射防护作用.结果显示:rhSOD和PEG-SOD均能明显提高CFU-S产率,其中rh SOD在照射前1h给药效果好,而PEG-SOD在照射前2～3h给药作用更为明显,化学修饰后SOD血浆半衰期延长,将使给药时机及药物作用持续时间得到改善.
The inhibition of hematopiesis that results from the irradiation of hematopoietic stemcells is one of the main cause of death in acute radiation diseases.Hematopoietic stem cells from irradiated donor mice injected with recombinant human superoxide dismutase(rh SOD) or polyethylene glycol (PEG) modified rh SOD(PEG-SOD)were transferred to irradiated host mice. Colony formation in host spleen was investigated. The results showed that rh SOD andPEG-SOD administered before 66Co irradiation had protective effect on the hematopoietic stem cells. Therh SOD had a maximal effect when administered 1 hour before irradiation, while PEG-SOD showed a lateand prolonged effect, probably due to the slower absorption and longer half-life of PEG-SOD.
关键词
超氧化物歧化酶
修饰
造血干细胞
外源性
脾结节
<Keyword>Recombinant human superoxide dismutase
Modify
Hematopoletic stem cells
Spleen colonies of host mice
